ipomoea-endophytes

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說明

The diversity of endophytic fungi of the sand coast plant Ipomoea pes-caprae in Taiwan was investigated.

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版本

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如何引用

請注意，此資料集為舊版本。 研究者應依照以下指示引用此資源。:

Yeh Y, Kirschner R (2021): ipomoea-endophytes. v1.3. Taiwan Biodiversity Information Facility (TaiBIF). Dataset/Occurrence. https://ipt.taibif.tw/resource?r=ipomoea-endophytes&v=1.3

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 Taiwan Biodiversity Information Facility (TaiBIF)。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 42b98a7f-4252-42a0-a56e-069df3eb3a7d。 Taiwan Biodiversity Information Facility (TaiBIF) 發佈此資源，並經由Taiwan Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。

關鍵字

Occurrence; Specimen; Occurrence

聯絡資訊

Yu-Hung Yeh

出處

Postdoc researcher

National Taiwan University

TW

Roland Kirschner

元數據提供者 ●
出處 ●
使用者 ●
連絡人

Professor

National Taiwan University

TW

kirschner@ntu.edu.tw

http://orcid.org/0000-0003-4956-5662

地理涵蓋範圍

Taiwan, Taipei, Taichung

界定座標範圍	緯度南界經度西界 [19.145, 116.543], 緯度北界經度東界 [29.841, 128.672]

分類群涵蓋範圍

Fungi

計畫資料

無相關描述

計畫名稱	The effect of ex-situ preservation on the diversity of endophytic fungi in the coastal plant Ipomoea pes-caprae
辨識碼	MOST 108-2621-B-008 -001
經費來源	Ministry of Science & Technology Taiwan

參與計畫的人員:

Yu-Hung Yeh

作者

Roland Kirschner

研究主持人

取樣方法

One individual plant was collected per sampling (twice per year, representing the summer and the less hot seasons). Plants which were not conspicuously buried by sand during the collection time were removed with a trowel, individually placed in bags, returned to the laboratory and kept at 4°C until further processing for endophyte isolation within 48 hours after sampling. Freshly collected healthy plants were cut into fragments (leaves, stems, roots) and surface-sterilized. Three roots, stems as well as six leaves per each of three collected plants were randomly selected. Plant fragments were surface-sterilized under sterile conditions by agitation in 95% ethanol for 1 min, 6–12% sodium hypochlorite for 3 min (for stems) or 1.5 min (for roots and leaves), 95% ethanol for 0.5 min, and then rinsed in sterile water. All stems and roots were cut into six segments of approximately 1–2 cm, and each leaf into the petiole as well as three segments of ca. 0.6 cm diam. from the lamina. Three segments of stems and root and two segments of leaf lamina and the petiole were immediately placed onto malt extract agar (MEA) with 0.2% chloramphenicol. All isolates obtained from each plant sample were classified according to their morphological appearance into morphotypes. Representative isolates were identified to species as far as possible and deposited at the Bioresource and Collection Center, Hsinchu, Taiwan (BCRC). Dried cultures were deposited in the fungal specimen collection of the National Museum of Natural Science, Taichung, Taiwan (TNM).

研究範圍	The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City.
品質控管	Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008).

研究範圍

The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City.

品質控管

Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008).

方法步驟描述:

Field collection, light microscopy, cultivation, DNA isolation, PCR, sequencing, sequence analyses

收藏資料

蒐藏名稱	Herbarium and Botanical Garden, Department of Botany, National Museum of Natural Science, No. 1, Kuan-Chien. Road, Taichung 404, Taiwan, R.O.C..

標本保存方法	Dried

額外的詮釋資料

替代的識別碼	42b98a7f-4252-42a0-a56e-069df3eb3a7d
	https://ipt.taibif.tw/resource?r=ipomoea-endophytes