ipomoea-endophytes

Latest version published by Taiwan Biodiversity Information Facility (TaiBIF) on Mar 22, 2021 Taiwan Biodiversity Information Facility (TaiBIF)

The diversity of endophytic fungi of the sand coast plant Ipomoea pes-caprae in Taiwan was investigated.

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How to cite

Researchers should cite this work as follows:

Yeh Y, Kirschner R (2021): ipomoea-endophytes. v1.2. Taiwan Biodiversity Information Facility (TaiBIF). Dataset/Occurrence. https://ipt.taibif.tw/resource?r=ipomoea-endophytes&v=1.2

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The publisher and rights holder of this work is Taiwan Biodiversity Information Facility (TaiBIF). This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 42b98a7f-4252-42a0-a56e-069df3eb3a7d.  Taiwan Biodiversity Information Facility (TaiBIF) publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Taiwan Biodiversity Information Facility.

Keywords

Occurrence; Specimen

Contacts

Who created the resource:

Yu-Hung Yeh
Postdoc researcher
National Taiwan University TW
Roland Kirschner
Professor
National Taiwan University TW

Who can answer questions about the resource:

Roland Kirschner
Professor
National Taiwan University TW

Who filled in the metadata:

Roland Kirschner
Professor
National Taiwan University TW

Who else was associated with the resource:

User
Roland Kirschner

Geographic Coverage

Taiwan, Taipei, Taichung

Bounding Coordinates South West [19.145, 116.543], North East [29.841, 128.672]

Taxonomic Coverage

Fungi

Project Data

No Description available

Title The effect of ex-situ preservation on the diversity of endophytic fungi in the coastal plant Ipomoea pes-caprae
Identifier MOST 108-2621-B-008 -001
Funding Ministry of Science & Technology Taiwan

The personnel involved in the project:

Principal Investigator
Roland Kirschner
Author
Yu-Hung Yeh

Sampling Methods

One individual plant was collected per sampling (twice per year, representing the summer and the less hot seasons). Plants which were not conspicuously buried by sand during the collection time were removed with a trowel, individually placed in bags, returned to the laboratory and kept at 4°C until further processing for endophyte isolation within 48 hours after sampling. Freshly collected healthy plants were cut into fragments (leaves, stems, roots) and surface-sterilized. Three roots, stems as well as six leaves per each of three collected plants were randomly selected. Plant fragments were surface-sterilized under sterile conditions by agitation in 95% ethanol for 1 min, 6–12% sodium hypochlorite for 3 min (for stems) or 1.5 min (for roots and leaves), 95% ethanol for 0.5 min, and then rinsed in sterile water. All stems and roots were cut into six segments of approximately 1–2 cm, and each leaf into the petiole as well as three segments of ca. 0.6 cm diam. from the lamina. Three segments of stems and root and two segments of leaf lamina and the petiole were immediately placed onto malt extract agar (MEA) with 0.2% chloramphenicol. All isolates obtained from each plant sample were classified according to their morphological appearance into morphotypes. Representative isolates were identified to species as far as possible and deposited at the Bioresource and Collection Center, Hsinchu, Taiwan (BCRC). Dried cultures were deposited in the fungal specimen collection of the National Museum of Natural Science, Taichung, Taiwan (TNM).

Study Extent The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City.
Quality Control Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008).

Method step description:

  1. Field collection, light microscopy, cultivation, DNA isolation, PCR, sequencing, sequence analyses

Collection Data

Collection Name Herbarium and Botanical Garden, Department of Botany, National Museum of Natural Science, No. 1, Kuan-Chien. Road, Taichung 404, Taiwan, R.O.C..
Specimen preservation methods Dried

Additional Metadata

Alternative Identifiers 42b98a7f-4252-42a0-a56e-069df3eb3a7d
https://ipt.taibif.tw/resource?r=ipomoea-endophytes