Description
The diversity of endophytic fungi of the sand coast plant Ipomoea pes-caprae in Taiwan was investigated.
Enregistrements de données
Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 896 enregistrements.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Comment citer
Veuillez noter qu'il s'agit d'une ancienne version du jeu de données. Les chercheurs doivent citer cette ressource comme suit:
Yeh Y, Kirschner R (2021): ipomoea-endophytes. v1.3. Taiwan Biodiversity Information Facility (TaiBIF). Dataset/Occurrence. https://ipt.taibif.tw/resource?r=ipomoea-endophytes&v=1.3
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est Taiwan Biodiversity Information Facility (TaiBIF). Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 42b98a7f-4252-42a0-a56e-069df3eb3a7d. Taiwan Biodiversity Information Facility (TaiBIF) publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Taiwan Biodiversity Information Facility.
Mots-clé
Occurrence; Specimen; Occurrence
Contacts
- Créateur
- Fournisseur Des Métadonnées ●
- Créateur ●
- Utilisateur ●
- Personne De Contact
Couverture géographique
Taiwan, Taipei, Taichung
Enveloppe géographique | Sud Ouest [19,145, 116,543], Nord Est [29,841, 128,672] |
---|
Couverture taxonomique
Fungi
Données sur le projet
Pas de description disponible
Titre | The effect of ex-situ preservation on the diversity of endophytic fungi in the coastal plant Ipomoea pes-caprae |
---|---|
Identifiant | MOST 108-2621-B-008 -001 |
Financement | Ministry of Science & Technology Taiwan |
Les personnes impliquées dans le projet:
- Auteur
- Chercheur Principal
Méthodes d'échantillonnage
One individual plant was collected per sampling (twice per year, representing the summer and the less hot seasons). Plants which were not conspicuously buried by sand during the collection time were removed with a trowel, individually placed in bags, returned to the laboratory and kept at 4°C until further processing for endophyte isolation within 48 hours after sampling. Freshly collected healthy plants were cut into fragments (leaves, stems, roots) and surface-sterilized. Three roots, stems as well as six leaves per each of three collected plants were randomly selected. Plant fragments were surface-sterilized under sterile conditions by agitation in 95% ethanol for 1 min, 6–12% sodium hypochlorite for 3 min (for stems) or 1.5 min (for roots and leaves), 95% ethanol for 0.5 min, and then rinsed in sterile water. All stems and roots were cut into six segments of approximately 1–2 cm, and each leaf into the petiole as well as three segments of ca. 0.6 cm diam. from the lamina. Three segments of stems and root and two segments of leaf lamina and the petiole were immediately placed onto malt extract agar (MEA) with 0.2% chloramphenicol. All isolates obtained from each plant sample were classified according to their morphological appearance into morphotypes. Representative isolates were identified to species as far as possible and deposited at the Bioresource and Collection Center, Hsinchu, Taiwan (BCRC). Dried cultures were deposited in the fungal specimen collection of the National Museum of Natural Science, Taichung, Taiwan (TNM).
Etendue de l'étude | The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City. |
---|---|
Contrôle qualité | Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008). |
Description des étapes de la méthode:
- Field collection, light microscopy, cultivation, DNA isolation, PCR, sequencing, sequence analyses
Données de collection
Nom de la collection | Herbarium and Botanical Garden, Department of Botany, National Museum of Natural Science, No. 1, Kuan-Chien. Road, Taichung 404, Taiwan, R.O.C.. |
---|
Méthode de conservation des spécimens | Dried |
---|
Métadonnées additionnelles
Identifiants alternatifs | 42b98a7f-4252-42a0-a56e-069df3eb3a7d |
---|---|
https://ipt.taibif.tw/resource?r=ipomoea-endophytes |