ipomoea-endophytes

DwC ファイルとしてのデータ	ダウンロード 896 レコード English で (19 KB) - 更新頻度: unknown
EML ファイルとしてのメタデータ	ダウンロード English で (9 KB)
RTF ファイルとしてのメタデータ	ダウンロード English で (9 KB)

説明

The diversity of endophytic fungi of the sand coast plant Ipomoea pes-caprae in Taiwan was investigated.

データレコード

このオカレンス（観察データと標本) リソース内のデータは、1 つまたは複数のデータテーブルとして生物多様性データを共有するための標準化された形式であるダーウィンコアアーカイブ (DwC-A) として公開されています。コアデータテーブルには、896 レコードが含まれています。

この IPT はデータをアーカイブし、データリポジトリとして機能します。データとリソースのメタデータは、ダウンロードセクションからダウンロードできます。バージョンテーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。

バージョン

次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。

引用方法

注意してください、これは、古いバージョンのデータセットです。 研究者はこの研究内容を以下のように引用する必要があります。:

Yeh Y, Kirschner R (2021): ipomoea-endophytes. v1.3. Taiwan Biodiversity Information Facility (TaiBIF). Dataset/Occurrence. https://ipt.taibif.tw/resource?r=ipomoea-endophytes&v=1.3

権利

研究者は権利に関する下記ステートメントを尊重する必要があります。:

パブリッシャーとライセンス保持者権利者は Taiwan Biodiversity Information Facility (TaiBIF)。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF登録

このリソースをはGBIF と登録されており GBIF UUID: 42b98a7f-4252-42a0-a56e-069df3eb3a7dが割り当てられています。 Taiwan Biodiversity Information Facility によって承認されたデータパブリッシャーとして GBIF に登録されているTaiwan Biodiversity Information Facility (TaiBIF) が、このリソースをパブリッシュしました。

キーワード

Occurrence; Specimen; Occurrence

連絡先

Yu-Hung Yeh

最初のデータ採集者

Postdoc researcher

National Taiwan University

TW

Roland Kirschner

メタデータ提供者 ●
最初のデータ採集者 ●
データ利用者 ●
連絡先

Professor

National Taiwan University

TW

kirschner@ntu.edu.tw

http://orcid.org/0000-0003-4956-5662

地理的範囲

Taiwan, Taipei, Taichung

座標（緯度経度）	南西 [19.145, 116.543], 北東 [29.841, 128.672]

生物分類学的範囲

Fungi

プロジェクトデータ

説明がありません

タイトル	The effect of ex-situ preservation on the diversity of endophytic fungi in the coastal plant Ipomoea pes-caprae
識別子	MOST 108-2621-B-008 -001
ファンデイング	Ministry of Science & Technology Taiwan

プロジェクトに携わる要員:

Yu-Hung Yeh

論文著者

Roland Kirschner

研究代表者

収集方法

One individual plant was collected per sampling (twice per year, representing the summer and the less hot seasons). Plants which were not conspicuously buried by sand during the collection time were removed with a trowel, individually placed in bags, returned to the laboratory and kept at 4°C until further processing for endophyte isolation within 48 hours after sampling. Freshly collected healthy plants were cut into fragments (leaves, stems, roots) and surface-sterilized. Three roots, stems as well as six leaves per each of three collected plants were randomly selected. Plant fragments were surface-sterilized under sterile conditions by agitation in 95% ethanol for 1 min, 6–12% sodium hypochlorite for 3 min (for stems) or 1.5 min (for roots and leaves), 95% ethanol for 0.5 min, and then rinsed in sterile water. All stems and roots were cut into six segments of approximately 1–2 cm, and each leaf into the petiole as well as three segments of ca. 0.6 cm diam. from the lamina. Three segments of stems and root and two segments of leaf lamina and the petiole were immediately placed onto malt extract agar (MEA) with 0.2% chloramphenicol. All isolates obtained from each plant sample were classified according to their morphological appearance into morphotypes. Representative isolates were identified to species as far as possible and deposited at the Bioresource and Collection Center, Hsinchu, Taiwan (BCRC). Dried cultures were deposited in the fungal specimen collection of the National Museum of Natural Science, Taichung, Taiwan (TNM).

Study Extent	The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City.
Quality Control	Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008).

Study Extent

The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City.

Quality Control

Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008).

Method step description:

Field collection, light microscopy, cultivation, DNA isolation, PCR, sequencing, sequence analyses

コレクションデータ

コレクション名	Herbarium and Botanical Garden, Department of Botany, National Museum of Natural Science, No. 1, Kuan-Chien. Road, Taichung 404, Taiwan, R.O.C..

標本保存方法	Dried

追加のメタデータ

代替識別子	42b98a7f-4252-42a0-a56e-069df3eb3a7d
	https://ipt.taibif.tw/resource?r=ipomoea-endophytes