ipomoea-endophytes

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Описание

The diversity of endophytic fungi of the sand coast plant Ipomoea pes-caprae in Taiwan was investigated.

Записи данных

Данные этого occurrence ресурса были опубликованы в виде Darwin Core Archive (DwC-A), который является стандартным форматом для обмена данными о биоразнообразии в виде набора из одной или нескольких таблиц. Основная таблица данных содержит 896 записей.

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Версии

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Как оформить ссылку

Пожалуйста, имейте в виду, это старая версия набора данных. Исследователи должны дать ссылку на эту работу следующим образом:

Yeh Y, Kirschner R (2021): ipomoea-endophytes. v1.3. Taiwan Biodiversity Information Facility (TaiBIF). Dataset/Occurrence. https://ipt.taibif.tw/resource?r=ipomoea-endophytes&v=1.3

Права

Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является Taiwan Biodiversity Information Facility (TaiBIF). This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 42b98a7f-4252-42a0-a56e-069df3eb3a7d. Taiwan Biodiversity Information Facility (TaiBIF) отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Taiwan Biodiversity Information Facility.

Ключевые слова

Occurrence; Specimen; Occurrence

Контакты

Yu-Hung Yeh

Originator

Postdoc researcher

National Taiwan University

TW

Roland Kirschner

Metadata Provider ●
Originator ●
User ●
Point Of Contact

Professor

National Taiwan University

TW

kirschner@ntu.edu.tw

http://orcid.org/0000-0003-4956-5662

Географический охват

Taiwan, Taipei, Taichung

Ограничивающие координаты	Юг Запад [19,145, 116,543], Север Восток [29,841, 128,672]

Таксономический охват

Fungi

Данные проекта

Описание отсутсвует

Название	The effect of ex-situ preservation on the diversity of endophytic fungi in the coastal plant Ipomoea pes-caprae
Идентификатор	MOST 108-2621-B-008 -001
Финансирование	Ministry of Science & Technology Taiwan

Исполнители проекта:

Yu-Hung Yeh

Author

Roland Kirschner

Principal Investigator

Методы сбора

One individual plant was collected per sampling (twice per year, representing the summer and the less hot seasons). Plants which were not conspicuously buried by sand during the collection time were removed with a trowel, individually placed in bags, returned to the laboratory and kept at 4°C until further processing for endophyte isolation within 48 hours after sampling. Freshly collected healthy plants were cut into fragments (leaves, stems, roots) and surface-sterilized. Three roots, stems as well as six leaves per each of three collected plants were randomly selected. Plant fragments were surface-sterilized under sterile conditions by agitation in 95% ethanol for 1 min, 6–12% sodium hypochlorite for 3 min (for stems) or 1.5 min (for roots and leaves), 95% ethanol for 0.5 min, and then rinsed in sterile water. All stems and roots were cut into six segments of approximately 1–2 cm, and each leaf into the petiole as well as three segments of ca. 0.6 cm diam. from the lamina. Three segments of stems and root and two segments of leaf lamina and the petiole were immediately placed onto malt extract agar (MEA) with 0.2% chloramphenicol. All isolates obtained from each plant sample were classified according to their morphological appearance into morphotypes. Representative isolates were identified to species as far as possible and deposited at the Bioresource and Collection Center, Hsinchu, Taiwan (BCRC). Dried cultures were deposited in the fungal specimen collection of the National Museum of Natural Science, Taichung, Taiwan (TNM).

Охват исследования	The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City.
Контроль качества	Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008).

Охват исследования

The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City.

Контроль качества

Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008).

Описание этапа методики:

Field collection, light microscopy, cultivation, DNA isolation, PCR, sequencing, sequence analyses

Данные коллекции

Название коллекции	Herbarium and Botanical Garden, Department of Botany, National Museum of Natural Science, No. 1, Kuan-Chien. Road, Taichung 404, Taiwan, R.O.C..

Методы сохранения образцов	Dried

Дополнительные метаданные

Альтернативные идентификаторы	42b98a7f-4252-42a0-a56e-069df3eb3a7d
	https://ipt.taibif.tw/resource?r=ipomoea-endophytes