ipomoea-endophytes

Descrição

Registros de Dados

Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 78 registros.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Lembre-se, esta é uma versão antiga do dataset.  Pesquisadores deveriam citar esta obra da seguinte maneira:

Yeh Y, Kirschner R (2021): ipomoea-endophytes. v1.2. Taiwan Biodiversity Information Facility (TaiBIF). Dataset/Occurrence. https://ipt.taibif.tw/resource?r=ipomoea-endophytes&v=1.2

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é Taiwan Biodiversity Information Facility (TaiBIF). This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 42b98a7f-4252-42a0-a56e-069df3eb3a7d.  Taiwan Biodiversity Information Facility (TaiBIF) publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Taiwan Biodiversity Information Facility.

Palavras-chave

Occurrence; Specimen

Contatos

Yu-Hung Yeh

• Originador

Postdoc researcher

National Taiwan University

TW

Roland Kirschner

• Provedor Dos Metadados ●
• Originador ●
• Usuário ●
• Ponto De Contato

Professor

National Taiwan University

TW

kirschner@ntu.edu.tw

http://orcid.org/0000-0003-4956-5662

Cobertura Geográfica

Taiwan, Taipei, Taichung

Cobertura Taxonômica

Fungi

Dados Sobre o Projeto

Nenhuma descrição disponível

O pessoal envolvido no projeto:

Métodos de Amostragem

One individual plant was collected per sampling (twice per year, representing the summer and the less hot seasons). Plants which were not conspicuously buried by sand during the collection time were removed with a trowel, individually placed in bags, returned to the laboratory and kept at 4°C until further processing for endophyte isolation within 48 hours after sampling. Freshly collected healthy plants were cut into fragments (leaves, stems, roots) and surface-sterilized. Three roots, stems as well as six leaves per each of three collected plants were randomly selected. Plant fragments were surface-sterilized under sterile conditions by agitation in 95% ethanol for 1 min, 6–12% sodium hypochlorite for 3 min (for stems) or 1.5 min (for roots and leaves), 95% ethanol for 0.5 min, and then rinsed in sterile water. All stems and roots were cut into six segments of approximately 1–2 cm, and each leaf into the petiole as well as three segments of ca. 0.6 cm diam. from the lamina. Three segments of stems and root and two segments of leaf lamina and the petiole were immediately placed onto malt extract agar (MEA) with 0.2% chloramphenicol. All isolates obtained from each plant sample were classified according to their morphological appearance into morphotypes. Representative isolates were identified to species as far as possible and deposited at the Bioresource and Collection Center, Hsinchu, Taiwan (BCRC). Dried cultures were deposited in the fungal specimen collection of the National Museum of Natural Science, Taichung, Taiwan (TNM).

Descrição dos passos do método:

1. Field collection, light microscopy, cultivation, DNA isolation, PCR, sequencing, sequence analyses

Dados de Coleção

Metadados Adicionais