Descripción
The diversity of endophytic fungi of the sand coast plant Ipomoea pes-caprae in Taiwan was investigated.
Registros
Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 78 registros.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
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¿Cómo referenciar?
Por favor, tenga en cuenta que ésta es una versión antigua del conjunto de datos. Los usuarios deben citar este trabajo de la siguiente manera:
Yeh Y, Kirschner R (2021): ipomoea-endophytes. v1.2. Taiwan Biodiversity Information Facility (TaiBIF). Dataset/Occurrence. https://ipt.taibif.tw/resource?r=ipomoea-endophytes&v=1.2
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es Taiwan Biodiversity Information Facility (TaiBIF). Este trabajo está autorizado bajo una Licencia Creative Commons Atribución/Reconocimiento 4.0 Internacional (CC-BY) 4.0.
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 42b98a7f-4252-42a0-a56e-069df3eb3a7d. Taiwan Biodiversity Information Facility (TaiBIF) publica este recurso y está registrado en GBIF como un publicador de datos avalado por Taiwan Biodiversity Information Facility.
Palabras clave
Occurrence; Specimen
Contactos
- Originador
- Proveedor De Los Metadatos ●
- Originador ●
- Usuario ●
- Punto De Contacto
http://orcid.org/0000-0003-4956-5662
Cobertura geográfica
Taiwan, Taipei, Taichung
| Coordenadas límite | Latitud Mínima Longitud Mínima [19,145, 116,543], Latitud Máxima Longitud Máxima [29,841, 128,672] |
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Cobertura taxonómica
Fungi
Datos del proyecto
No hay descripción disponible
| Título | The effect of ex-situ preservation on the diversity of endophytic fungi in the coastal plant Ipomoea pes-caprae |
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| Identificador | MOST 108-2621-B-008 -001 |
| Fuentes de Financiación | Ministry of Science & Technology Taiwan |
Personas asociadas al proyecto:
- Autor
- Investigador Principal
Métodos de muestreo
One individual plant was collected per sampling (twice per year, representing the summer and the less hot seasons). Plants which were not conspicuously buried by sand during the collection time were removed with a trowel, individually placed in bags, returned to the laboratory and kept at 4°C until further processing for endophyte isolation within 48 hours after sampling. Freshly collected healthy plants were cut into fragments (leaves, stems, roots) and surface-sterilized. Three roots, stems as well as six leaves per each of three collected plants were randomly selected. Plant fragments were surface-sterilized under sterile conditions by agitation in 95% ethanol for 1 min, 6–12% sodium hypochlorite for 3 min (for stems) or 1.5 min (for roots and leaves), 95% ethanol for 0.5 min, and then rinsed in sterile water. All stems and roots were cut into six segments of approximately 1–2 cm, and each leaf into the petiole as well as three segments of ca. 0.6 cm diam. from the lamina. Three segments of stems and root and two segments of leaf lamina and the petiole were immediately placed onto malt extract agar (MEA) with 0.2% chloramphenicol. All isolates obtained from each plant sample were classified according to their morphological appearance into morphotypes. Representative isolates were identified to species as far as possible and deposited at the Bioresource and Collection Center, Hsinchu, Taiwan (BCRC). Dried cultures were deposited in the fungal specimen collection of the National Museum of Natural Science, Taichung, Taiwan (TNM).
| Área de Estudio | The study was conducted in northern and central Taiwan; the datasets covers natural vegetation at sand coast of New Taipei City (Bali District and Shihmen District) and Taichung City (Daan District and North District)and artificial plantations in the botanical gardens of Taipei City and Taichung City. |
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| Control de Calidad | Surface-sterilization was adjusted whenever endophytes are to be isolated from a hitherto uninvestigated plant species. We optimized the methods for surface sterilization for the different plant parts of I. pes-caprae. The effectiveness was further controlled regularly by the imprint technique, i.d. by temporarily pressing the surface-sterilized plant fragments onto control media. Only if no growth occured on these control media, but in the media with plant fragments, then the surface sterilization procedure was neither too weak nor too rigorous (Rodrigruez et al. 2008). |
Descripción de la metodología paso a paso:
- Field collection, light microscopy, cultivation, DNA isolation, PCR, sequencing, sequence analyses
Datos de la colección
| Nombre de la Colección | Herbarium and Botanical Garden, Department of Botany, National Museum of Natural Science, No. 1, Kuan-Chien. Road, Taichung 404, Taiwan, R.O.C.. |
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| Métodos de preservación de los ejemplares | Secado |
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Metadatos adicionales
| Identificadores alternativos | 42b98a7f-4252-42a0-a56e-069df3eb3a7d |
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| https://ipt.taibif.tw/resource?r=ipomoea-endophytes |