說明
This project documents bird specimens collected across Taiwan from 1991 to 2012, as part of an effort to preserve avian genetic resources and contribute to the knowledge of biodiversity in Taiwan and globally. Specimens were obtained from naturally deceased individuals and non-lethal sources such as window collisions, banding mortality, and rescue center mortalities. These were donated by a range of contributors, including private individuals, ornithological societies, universities, conservation agencies, wildlife rescue centers and others. From 2012 to 2013, specimens underwent wet lab procedures at the National Museum of Natural Science (NMNS), Taichung. Muscle tissue was sampled under sterile conditions, and DNA was extracted and sequenced targeting the mitochondrial cytochrome c oxidase subunit I (COI) gene, a widely used barcode marker for avian species identification. The dataset comprises 191 records, covering a diverse range of bird species from multiple orders and families, aligned with the 2023 TWBF Checklist of the Birds of Taiwan (Link to checklist: https://www.bird.org.tw/sites/default/files/field/file/download/The%202023%20TWBF%20Checklist%20of%20the%20Birds%20of%20Taiwan%2020240624ed.pdf). Most records are georeferenced to sites across Taiwan. Sequencing data are publicly available via NCBI GenBank, with accession numbers ranging from PV770366 to PV770556. This dataset contributes both morphological and molecular resources to the scientific community, supporting avian taxonomy, DNA barcoding, and long-term conservation research. All bird specimens were collected under the Ornithology Collection of the National Museum of Natural Science (NMNS) and are curated to ensure high standards of preservation, data quality, and accessibility for future research.
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 191 筆紀錄。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Hoh D, Yao C (2026). NMNS Avian DNA Barcode Project. Version 1.1. National Museum of Natural Science. Occurrence dataset. https://ipt.taibif.tw/resource?r=nmns_avian_dna_barcode_project&v=1.1
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 National Museum of Natural Science。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 586c3f2c-4bb4-4581-b33e-5bfc9a9c9bd5。 National Museum of Natural Science 發佈此資源，並經由Taiwan Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence; Specimen
聯絡資訊
- 出處
https://orcid.org/0000-0002-7810-1034
- 元數據提供者 ●
- 連絡人
地理涵蓋範圍
Taiwan
| 界定座標範圍 | 緯度南界 經度西界 [21.952, 118.367], 緯度北界 經度東界 [25.485, 122.106] |
|---|
分類群涵蓋範圍
無相關描述
| Kingdom | Animalia |
|---|---|
| Phylum | Chordata |
| Class | Aves |
| Order | Suliformes, Cuculiformes, Anseriformes, Galliformes, Passeriformes, Pelecaniformes, Falconiformes, Accipitriformes, Gruiformes, Columbiformes, Apodiformes, Charadriiformes, Bucerotiformes, Podicipediformes, Strigiformes, Coraciiformes, Piciformes |
| Family | Laridae, Corvidae, Upupidae, Phylloscopidae, Columbidae, Motacillidae, Muscicapidae, Cuculidae, Podicipedidae, Pycnonotidae, Glareolidae, Picidae, Sturnidae, Alcedinidae, Scolopacidae, Sterna, Reguliidae, Charadriidae, Threskiornithidae, Accipitridae, Campephagidae, Hirundinidae, Strigidae, Phasianidae, Falconidae, Timaliidae, Apodidae, Turdidae, Rallidae, Tytonidae, Sulidae, Turnicidae, Laniidae, Paridae, Ardeidae, Locustellidae, Oriolidae, Dicruridae, Anatidae, Pittidae |
時間涵蓋範圍
| 起始日期 / 結束日期 | 0029-09-12 / 0028-04-03 |
|---|
計畫資料
無相關描述
| 計畫名稱 | NMNS Avian DNA Barcode Project |
|---|---|
| 經費來源 | This project is supported by subsidies from the Ministry of Agriculture, Forestry, and the Nature Conservation Agency, Taiwan, for the projects titled "Operation of the Cryopreservation Genetic Material Repository and Utilization of Genetic Material" (Part 2 of 3) in 2012 and (Part 3 of 3) in 2013. |
取樣方法
Bird specimens were primarily obtained from dead individuals found in the field across Taiwan. These specimens were donated by a variety of sources, including private individuals, ornithological societies, airport authorities, universities, government conservation and research agencies, national parks, and bird conservation organizations. A smaller proportion originated from window collisions, mortalities during bird banding research, or birds that did not survive rescue efforts at wildlife rescue and rehabilitation centers.
| 研究範圍 | This study focuses on avian specimens collected across Taiwan. The majority of specimens were sourced from naturally deceased individuals found in the field, with additional contributions from bird collisions, banding mortalities, and unsuccessful wildlife rescue cases. Sampling and genetic analysis were performed at the National Museum of Natural Science (NMNS), Taichung. The dataset encompasses a temporal span of collections work from 1991 to 2012, and molecular work from 2012 to 2013, primarily focusing on the mitochondrial COI region for species identification and genetic resource preservation. |
|---|---|
| 品質控管 | The occurrence data of the bird specimens collected was formatted into the Darwin Core format (Darwin Core Maintenance Group, 2023) and validated using GBIF's Data Validator tool (https://www.gbif.org/tools/data-validator). |
方法步驟描述:
- Sample collection: Each specimen was sampled individually, with all procedures conducted by personnel wearing masks and freshly disinfected rubber gloves. Prior to sampling, the workbench, all equipment (including gloves and the drill machine), and the bone sampling area were thoroughly cleaned using a 10% bleach solution. A sterile stainless steel instrument, autoclaved prior to use, was employed to collect muscle samples weighing between 10 and 25 mg. To minimize the risk of cross-contamination, each drill bit was used only once. Following collection, all samples were promptly stored at –20 °C until further processing.
- DNA Extraction: DNA extraction of the muscle samples was performed at a dedicated workbench in the National Museum of Natural Science, Taichung. Prior to use, the bench surface was thoroughly sterilized using 75% alcohol followed by a 10% bleach solution. DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, 19300 Germantown Rd., Germantown, MD 20874, United States), following the manufacturer’s recommended protocol.
- Polymerase Chain Reaction (PCR) and DNA Sequencing: The extracted DNA was subsequently amplified using PCR to target specific regions of mitochondrial DNA, including the control region and the cytochrome c oxidase subunit I (COI) gene. Because the specimens were aged and had been stored under conditions of elevated temperature and humidity, the DNA was expected to be significantly degraded. To accommodate this, primers were designed to amplify short fragments ranging from 200 to 300 base pairs. PCR was conducted in a 20 μL reaction mixture containing 10–80 ng of template DNA, 0.2 mM of dNTPs, 0.1 μM of each primer, 0.5 units of Ex Taq DNA Polymerase (TaKaRa), and 1× PCR buffer. Amplification was carried out using a Biometra TProfessional thermocycler with the following thermal profile: an initial denaturation at 95 °C for 5 minutes; 40 cycles of 30 seconds at 94 °C, 30 seconds at the primer-specific annealing temperature, and 30 seconds at 72 °C; followed by a final extension at 72 °C for 5 minutes. PCR products were visualized by electrophoresis on a 2% agarose gel to confirm amplification success. Positive amplicons were purified using the QIAquick PCR Purification Kit (Qiagen), and direct sequencing was performed following the PRISM™ Ready Reaction Dye Deoxy Terminator Protocol. Sequencing was conducted on an ABI 3730XL Genetic Analyzer (Applied Biosystems Inc., Foster City, CA, USA).
收藏資料
| 蒐藏名稱 | Ornithology Collection |
|---|
額外的詮釋資料
| 替代的識別碼 | 586c3f2c-4bb4-4581-b33e-5bfc9a9c9bd5 |
|---|---|
| https://ipt.taibif.tw/resource?r=nmns_avian_dna_barcode_project |