NMNS Avian DNA Barcode Project

Enregistrements de données

Les données de cette ressource occurrence ont été publiées sous forme dune Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant quensemble dun ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 191 enregistrements.

Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.

Versions

Le tableau ci-dessous naffiche que les versions publiées de la ressource accessibles publiquement.

Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Hoh D, Yao C (2026). NMNS Avian DNA Barcode Project. Version 1.1. National Museum of Natural Science. Occurrence dataset. https://ipt.taibif.tw/resource?r=nmns_avian_dna_barcode_project&v=1.1

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est National Museum of Natural Science. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède lUUID GBIF suivante : 586c3f2c-4bb4-4581-b33e-5bfc9a9c9bd5.  National Museum of Natural Science publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec lapprobation du Taiwan Biodiversity Information Facility.

Mots-clé

Occurrence; Specimen

Contacts

Couverture géographique

Taiwan

Couverture taxonomique

Pas de description disponible

Couverture temporelle

Données sur le projet

Pas de description disponible

Méthodes déchantillonnage

Bird specimens were primarily obtained from dead individuals found in the field across Taiwan. These specimens were donated by a variety of sources, including private individuals, ornithological societies, airport authorities, universities, government conservation and research agencies, national parks, and bird conservation organizations. A smaller proportion originated from window collisions, mortalities during bird banding research, or birds that did not survive rescue efforts at wildlife rescue and rehabilitation centers.

Description des étapes de la méthode:

1. Sample collection: Each specimen was sampled individually, with all procedures conducted by personnel wearing masks and freshly disinfected rubber gloves. Prior to sampling, the workbench, all equipment (including gloves and the drill machine), and the bone sampling area were thoroughly cleaned using a 10% bleach solution. A sterile stainless steel instrument, autoclaved prior to use, was employed to collect muscle samples weighing between 10 and 25 mg. To minimize the risk of cross-contamination, each drill bit was used only once. Following collection, all samples were promptly stored at –20 °C until further processing.
2. DNA Extraction: DNA extraction of the muscle samples was performed at a dedicated workbench in the National Museum of Natural Science, Taichung. Prior to use, the bench surface was thoroughly sterilized using 75% alcohol followed by a 10% bleach solution. DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, 19300 Germantown Rd., Germantown, MD 20874, United States), following the manufacturer’s recommended protocol.
3. Polymerase Chain Reaction (PCR) and DNA Sequencing: The extracted DNA was subsequently amplified using PCR to target specific regions of mitochondrial DNA, including the control region and the cytochrome c oxidase subunit I (COI) gene. Because the specimens were aged and had been stored under conditions of elevated temperature and humidity, the DNA was expected to be significantly degraded. To accommodate this, primers were designed to amplify short fragments ranging from 200 to 300 base pairs. PCR was conducted in a 20 μL reaction mixture containing 10–80 ng of template DNA, 0.2 mM of dNTPs, 0.1 μM of each primer, 0.5 units of Ex Taq DNA Polymerase (TaKaRa), and 1× PCR buffer. Amplification was carried out using a Biometra TProfessional thermocycler with the following thermal profile: an initial denaturation at 95 °C for 5 minutes; 40 cycles of 30 seconds at 94 °C, 30 seconds at the primer-specific annealing temperature, and 30 seconds at 72 °C; followed by a final extension at 72 °C for 5 minutes. PCR products were visualized by electrophoresis on a 2% agarose gel to confirm amplification success. Positive amplicons were purified using the QIAquick PCR Purification Kit (Qiagen), and direct sequencing was performed following the PRISM™ Ready Reaction Dye Deoxy Terminator Protocol. Sequencing was conducted on an ABI 3730XL Genetic Analyzer (Applied Biosystems Inc., Foster City, CA, USA).

Données de collection

Métadonnées additionnelles