ipomoea-endophytes

Description

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 896 records.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Please be aware, this is an old version of the dataset.  Researchers should cite this work as follows:

Yeh Y, Kirschner R (2021): ipomoea-endophytes. v1.3. Taiwan Biodiversity Information Facility (TaiBIF). Dataset/Occurrence. https://ipt.taibif.tw/resource?r=ipomoea-endophytes&v=1.3

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is Taiwan Biodiversity Information Facility (TaiBIF). This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 42b98a7f-4252-42a0-a56e-069df3eb3a7d.  Taiwan Biodiversity Information Facility (TaiBIF) publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Taiwan Biodiversity Information Facility.

Keywords

Occurrence; Specimen; Occurrence

Contacts

Yu-Hung Yeh

• Originator

Postdoc researcher

National Taiwan University

TW

Roland Kirschner

• Metadata Provider ●
• Originator ●
• User ●
• Point Of Contact

Professor

National Taiwan University

TW

kirschner@ntu.edu.tw

http://orcid.org/0000-0003-4956-5662

Geographic Coverage

Taiwan, Taipei, Taichung

Taxonomic Coverage

Fungi

Project Data

No Description available

The personnel involved in the project:

Sampling Methods

One individual plant was collected per sampling (twice per year, representing the summer and the less hot seasons). Plants which were not conspicuously buried by sand during the collection time were removed with a trowel, individually placed in bags, returned to the laboratory and kept at 4°C until further processing for endophyte isolation within 48 hours after sampling. Freshly collected healthy plants were cut into fragments (leaves, stems, roots) and surface-sterilized. Three roots, stems as well as six leaves per each of three collected plants were randomly selected. Plant fragments were surface-sterilized under sterile conditions by agitation in 95% ethanol for 1 min, 6–12% sodium hypochlorite for 3 min (for stems) or 1.5 min (for roots and leaves), 95% ethanol for 0.5 min, and then rinsed in sterile water. All stems and roots were cut into six segments of approximately 1–2 cm, and each leaf into the petiole as well as three segments of ca. 0.6 cm diam. from the lamina. Three segments of stems and root and two segments of leaf lamina and the petiole were immediately placed onto malt extract agar (MEA) with 0.2% chloramphenicol. All isolates obtained from each plant sample were classified according to their morphological appearance into morphotypes. Representative isolates were identified to species as far as possible and deposited at the Bioresource and Collection Center, Hsinchu, Taiwan (BCRC). Dried cultures were deposited in the fungal specimen collection of the National Museum of Natural Science, Taichung, Taiwan (TNM).

Method step description:

1. Field collection, light microscopy, cultivation, DNA isolation, PCR, sequencing, sequence analyses

Collection Data

Additional Metadata